TY  - BOOK
AU  - Ahmed Hassan Mohamed Elsaid
AU  - Gamal Helmy Salem , 
AU  - Mahmoud Essam Hatem , 
AU  - Mohamed Kamal Refai , 
TI  - Studies on applications of molecular biology techniques versus conventional methods for direct detection of salmonella in cattle in Egypt / 
PY  - 2018///
CY  - Cairo : 
PB  - Ahmed Hassan Mohamed Elsaid , 
KW  - PCR
KW  - Real-Time PCR
KW  - Salmonella spp
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology; Issued also as CD
N2  - A total of 179 samples of fecal swabs from diarrhea suffering cattle were randomly collected from slaughterhouses as well as dairy farms. The conventional cultural, biochemical and serological methods for detection and identification of Salmonella in fecal swabs were applied and the results were compared with those obtained by molecular screening assays using conventional PCR and Real-Time PCR techniques. The obtained results revealed that 9.1% of were positive for Salmonella species using conventional culture methods while 11.8% were positive by conventional PCR and 15.5% by using Real-Time PCR were found to be positive for Salmonella species. The results of the three methods were compared to each other. From the present work it could be concluded that the Real-Time PCR assay has highest sensitivity and specificity for detection of Salmonella species in dairy farms
ER  -