TY - BOOK AU - Sarah Sayed Ahmed Mohamed Elshami AU - Abdelwahed Mahmoud Hassan , AU - Alaa Eldin Abdelmouty Eissa , AU - Jakeen Kamal EIjakee , TI - Isolation and characterization of mycoplasmas from wild and earthen pond raised fishes in Egypt / PY - 2018/// CY - Cairo : PB - Sarah Sayed Ahmed Mohamed Elshami , KW - Mycoplasmas KW - PCR KW - Wild and earthen pond raised fishes N1 - Thesis (M.Sc.) - Cairo University -Faculty of Veterinary Medicine - Department of Microbiology; Issued also as CD N2 - A total of 299 samples were collected from 5 species of freshwater and brackishwater fish (from five organs: gills, liver, gas bladder, intestine and spleen) through 6 governorates. Forty two samples were positive for Mycoplasma and designated Mycoplasma species 1 (35 isolates) and Mycoplasma species 2 (7 isolates) according to the biochemical characterization, PCR test and consequent genetic blast allocation through NCBI Blast system.The two isolates were subjected to further identification by sequence analysis of the 16S rRNA gene which revealed the high similarity between the two isolates and the presence of 98 % similarity of them to some other Mycoplasma specially M. gallinarum and M. adleri based on nucleotide sequences comparison at the NCBI Blast System UR - http://172.23.153.220/th.pdf ER -