Staphylococcus aureus as a causative agent of subclinical mastitis in dairy farms with special reference to mrsa at Elgharbia governorate /
المكور العنقودى الذهبى كمسبب لالتهاب الضرع الكامن فى مزارع الالبان مع الاشاره الى المكورات العنقودية المقاومه للميثيسلين في محافظة الغربيه
Marwa Nabil Sayed Abdelaziz ; Supervised Hamdy Abdelaziz Elesawy , Salwa Ahmed Mohamed Aly , Gamal Ragab Hasab Elnaby
- Cairo : Marwa Nabil Sayed Abdelaziz , 2018
- 125 P. : charts , facsimils ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Food Hygiene and Control
Three hundred and fifty quarter milk samples were collected from apparently healthy cows at Al-Gharbia governorate, Egypt. Samples were tested using California Mastitis Test (CMT) and somatic cell count, of which 235 samples were detected as positive. Overall, 168 quarter milk samples (71.48%) were found to be contaminated with coagulase positive Staphylococcus aureus with a mean value of 64x103± 19x103cfu/ml. Isolated Staphylococcus aureus strains were tested for methicillin to identify Methicillin resistant strains (MRSA). Antibiotic sensitivity test was carried out by using two antibiotic disks against fourty six identified Staphylococcus aureus isolates. The obtained results indicated that resistance against cefoxitin was 34.78% and sensitivity was 65.22%, while the resistance against vancomycin was 26.08% and sensitivity was 73.92%. PCR technique was used to detect presence of mecA gene that coded for penicillin-binding protein 2a. The results on sixteen positive isolates which suspected to have mecA gene by antibiotic sensitivity test were 93.75%. The total of subclinical mastitis cases infected with MRSA was 31.11%. The results provided evidence that the presence of Staphylococcus aureus, as well as Methicillinresistant strains (MRSA) have become remarkably widespread in subclinical mastitic quarter milk samples. This calls for better control of the sources of milk contamination as well as spread of antimicrobial resistance organisms
California mastitis test Staphylococcus aureus Subclinical mastitis