TY  - BOOK
AU  - Shimaa Ahmed Mosaad Basha
AU  - Fatma Elrefaey , 
AU  - Sherif Naseh Amin , 
AU  - Yasser Hassan Elnahass , 
TI  - A pilot study of different morphological features in myeloproliferative neoplasms with calreticulin  mutations / 
PY  - 2018///
CY  - Cairo : 
PB  - Shimaa Ahmed Mosaad Basha , 
KW  - CALR
KW  - MPL
KW  - MPN
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology; Issued also as CD
N2  - Back ground Philadelphia chromosome{u2013}negative myeloproliferative neoplasms constitute a heterogeneous group of hematopoietic disorders manifested by a variety of morphologic changes in the bone marrow (BM).Calreticulin(CALR) is a multifunctional protein that binds Ca2+ions (a second messenger in signal transduction), rendering it inactive.The Ca2+ is bound with low affinity, but high capacity, and can be released on a signal.CALR is located in storage compartments associated with the endoplasmic reticulum. Aim of the work: The aim of this study is to describe and correlate the morphologic features ofmyeloproliferative neoplasms(MPNs) especially ET and PMF with CALR mutations. Patients and Methods: The study was conducted on 68 MPNs patients;41/68 ET ,24/68 PMF and 3/68 post ET-MF.CALR Mutations By High ReslutionMelting(HRM) Analysis and Bone marrow trephine biopsy were done. Results : CALR-mut ET patients were more likely to show dense clustering of megakaryocytes compared to CALR-wt patients 8/10 (80%) vs 13/31 (41.9%) (p = 0.036).CALR-mut  PMF showed megakaryocytes that more often exhibited nuclear hyperchromasia compared to CALR-wt PMF 3 / 4(75%) cases vs 11 / 20(55%) cases ( P = .0615). All post ET-MF patients showed variable megakaryocyte morphology. 3/4(75%) were JAK 2 positive .variable morphology included more abnormal bizzare ,hypolobated morphology
UR  - http://172.23.153.220/th.pdf
ER  -