TY  - BOOK
AU  - Samar Ahmed Mohamed Altohamy
AU  - Amal Mohammed Sayed , 
AU  - Eiman Mohammed Abdulrahman , 
AU  - Reham Hamed Abdelaziz , 
TI  - Loop-mediated isothermal amplification (LAMP) assay for rapid detection of Mycoplasma pneumoniae infection in paediatric Egyptian patients / 
PY  - 2018///
CY  - Cairo : 
PB  - Samar Ahmed Mohamed Altohamy , 
KW  - Assay for rapid detection
KW  - Isothermal amplification LAMP
KW  - Mycoplasma pneumoniae infection
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology; Issued also as CD
N2  - Currently, three methods are available for the routine diagnosis of infections caused by M. pneumoniae: culture, serology and nucleic acid amplification techniques. Culture is time-consuming, taking several weeks to produce results and is relatively insensitive (Loens et al., 2003b). Serological methods are insufficiently sensitive and require paired serum samples from the acute and convalescent phases of the disease, thus only allowing a retrospective diagnosis (Zhang et al., 2011). More rapid, higher sensitivity methods were therefore developed, one of them being the PCR for fragments of the P1 gene (Chaudhry et al., 2013). Previous studies described real-time PCR methods for the diagnosis of M. pneumoniae e.g. the 16S rRNA genes or the P1 adhesion gene (Hardegger et al., 2000). Also, ADP-ribosylating toxin gene encoding the CARDS (community-acquired respiratory distress syndrome) toxin for real-time PCR detection
ER  -