TY  - BOOK
AU  - Mona Magdy Saber Moawad
AU  - Ezz Eldin Eldenshary , 
AU  - Noha Nagah Nassar , 
AU  - Samia A. Shouman , 
TI  - Pharmacological study of the combinatorial cytotoxic effect of certain drugs in human colon cancer cell lines / 
PY  - 2018///
CY  - Cairo : 
PB  - Mona Magdy Saber Moawad , 
KW  - Cisplatin
KW  - CRC
KW  - Metformin
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Pharmacology and Toxicology; Issued also as CD
N2  - Introduction: Colorectal cancer (CRC) remains a leading cause of death worldwide.The use of cisplatin as an anti-cancer drug in CRC is correlated with severe adverse effects and drug resistance. Utilizing combination of anticancer agents can effectively kill cancer through multiple pathways; nonetheless, improvements in their delivery are needed. Nano-cubosomes, thanks to the advantages of their liquid crystalline porous nano-architecture and capability for multi-drug encapsulation, appear to be of interest as nanocarriers for anti-cancer therapies. Hence, delivering cisplatin, as nano-cubosomes, concomitantly with other chemo-dugs can lead to a rationally designed therapy for chemo-resistant cancers and might overcome problems associated with conventional cisplatin treatment. Aim: The aim of the present study was to construct nano-cubosomes bearing cisplatin, metformin and cisplatin-metformin combination and investigate their effect on proliferation in Caco-2 and HCT-116 cell lines, as well as, tumorigenesis-associated metabolic markers in HCT-116 colorectal cancer cell line. Materials and methods: Nano-cubosomes bearing either cisplatin alone, metformin alone or cisplatin-metformin combination were formulated using emulsification technique. The loaded nano-cubosomes were characterized in vitro and the optimized formulations were selected.Their cytotoxic effects were investigated by Sulphorhodamine-B (SRB) assayin Caco-2 and HCT-116 cells. In HCT-116 cellsthe AMPK/mTOR metabolic pathwaywas analyzed using ELISA technique.Protein levels of AKT were determined using western blotting. Glucose, ATP, NADPH oxidase, LDH, glutathione (GSH) and caspase-3 activity were measured spectrophotometrically
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ER  -