TY  - BOOK
AU  - Ahmed Salah Emira Aly
AU  - Abdelfattah Mohamed Attia , 
AU  - Nazmy Edward Seif , 
AU  - Reham Ali Dwedar , 
TI  - Multiplex PCR for detection of five selected carbapenemase genes in comparative study with modified-hodge test in gram-negative bacilli isolated from healthcare-associated infections among critically Ill patients / 
PY  - 2018///
CY  - Cairo : 
PB  - Ahmed Salah Emira Aly , 
KW  - Carbapenemase
KW  - Modified Hodge test
KW  - Silent Genes
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology; Issued also as CD
N2  - Background:Carbapenemase producing Gram-negative bacilli have been reported to be an important cause of hospital associated infections (HAIs), especially in critically ill patients with device associated infections (DAIs). Aim of the work: To estimate the extent of carbapenemase producing Gram-negative bacteria in critically ill patients from Kasr El-Aini, Cairo University hospitals and to compare the Modified-Hodge test (MHT) as a phenotypic confirmatory test with the PCR as a gold standard genotypic method for their diagnosis. Patients and methods:95 bacterial isolates from 75 critically ill patients were collected over a period of six months from November 2016 to April 2017 from Cairo university hospitals. These isolates were subjected to susceptibility testing for carbapenems as per CLSI guidelines and were further phenotypically screened for the production of carbapenemase by MHT, then were subjected to genotypic analysis by multiplex PCR
UR  - http://172.23.153.220/th.pdf
ER  -