TY  - BOOK
AU  - Buthainah Ahmed Sayed Salem
AU  - Mahmoud Dardiri  Elhariri , 
AU  - Nashwa Abdelsalam Ezz Eldeen , 
TI  - Microbiological and molecular diagnosis of pasteurella multocida in sheep / 
PY  - 2018///
CY  - Cairo : 
PB  - Buthainah Ahmed Sayed Salem , 
KW  - Microbiological
KW  - Pasteurella  multocida
KW  - PCR
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine- Department of Microbiology; Issued also as CD
N2  - Pasteurella multocida was isolated from nasal swabs of diseased animal , apparently healthy animal and lung sample of diseased and emergency slautered animal by three method one  was  by conventional ordinary  culture method and the  other were By applied for Two non-culturable methods depending on direct molecular detection on either examined sample or 24 hours preselective broth. Percent of isolation greatly different in each method as in conventional method culture  percent were 9.3%  from apparently healthy., 15.3% from pneumonic lungs of slaughtered animals, and 12 % nasal swabs of diseased animals with total recovery rate 13.0 %. While the Direct-PCR approaches While, by the Direct-PCR approaches. The direct PCR on samples detection percent were 16.0 % ,13.3 % & 22.0% from nasal swabs of healthy , diseased and pneumonic lung tissues, respectively .And  direct PCR on preselective broth detection percent were (12.0%) from apparently healthy, (24.0%) from diseased animals and( 48.6%) from emergency slaughtered sheep  and it is the highest detection rate (33.3%) of P. multocida in comparison to other two methods
ER  -