Hend Abdelhady Nooh Mahmood <br/><br/>
Detection of IDH1 and IDH2 mutations in denovo acute myeloid leukemia patients at National Cancer Institute, Cairo University /  
 في حالات سرطان الدم الميلودي الحاد الحديثة التشخيص  بالمعهد القومي للاورام - جامعة القاهرة  Isocitrate Dehydrogenase (IDH 1,2) كشف الطفرة الجينيه لجيني     
Hend Abdelhady Nooh Mahmood ; Supervised Ragia H. Badawy , Yasser  H. Elnahhas , Fatma A. Elrefaey 
 - Cairo :   Hend Abdelhady Nooh Mahmood ,   2019 
 - 187 P. :   charts ;   25cm  
<br/><br/>Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Clinical Pathology 
<br/><br/> Introduction: AML is characterized by the uncontrolled clonal proliferation of poorly differentiated cells of myeloblasts. Isocitrate dehydrogenases (IDH1&2) are enzymes involved in multiple  metabolic and epigenetic cellular processes. Mutations in IDH1 or IDH2 induce production of an oncometabolite, 2-hydroxyglutarate (R-2-HG) which leads to DNA hypermethylation, and abnormal differentiation. IDH mutations diversely affect prognosis of patients with AML based on the presence of other co-occurring genomic abnormalities.  Aim of the work : The aim of this study was to assess the frequency of  IDH1& IDH2 genes mutations in AML patients and evaluate their role in disease prognosis.  Patients and Methods: We analyzed IDH mutations in 70 denovo AML patients (exon 4 for IDH1& exon 4 for IDH2) using High Resolution Melting (HRM) curve analysis 
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<br/><br/><label>Subjects--Index Terms: </label>AML (Acute Myeloid Leukemia) IDH (Isociterate Dehydrogenase)  Karyotype