TY  - BOOK
AU  - Heba Elsayed Mahfouz Elzorkany
AU  - Salwa Thabet , 
AU  - Taher Salah Eldin , 
AU  - Tareq Youssef ِAbdelwahed , 
TI  - Efficiency of biocompatible quantum dots for cellular imaging using confocal laser scanning microscope / 
PY  - 2019///
CY  - Cairo : 
PB  - Heba Elsayed Mahfouz Elzorkany , 
KW  - Biocompatible quantum dots
KW  - Confocal laser scanning microscope
KW  - Quantum dots (QDs)
N1  - Thesis (Ph.D.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Application in Environmental Metrology Photochemistry and Agriculture; Issued also as CD
N2  - Quantum dots (QDs) are zero-dimensional systems wherein charge carriers (i.e., electrons and holes) are confined in all three  dimensions. QDs are a very interesting nanomaterial with unique characteristics, which could help in many clinical and  pharmaceutical purposes. In this study, we aimed to investigate the efficiency of silica-coated CdSe/ZnS (CdSe/ZnS-SiO NCs) and Graphene 2 nanocrystals (GQDs) for imaging purposes. CdSe quantum dots (QDs) were synthesized by organometallic routes and were coated with ZnS shell by injecting solutions of diethylzinc (Zn (Et) ₂) and hexamethyldislathiane ((TMS)₂ S) as precursors for zinc and sulfur ions respectively. On the other hand, GQDs were synthesized via glucose pyrolysis. Then, the prepared NCs overcoated with silica using tetraethyl orthosilicate (TEOS) as a silica precursor. QDs were characterized by UV-Vis absorption,  emission spectroscopy TEM, XRD, and DLS. The biocompatibility of silica-coated QDs was tested by evaluating mitochondrial activity and alkaline comet assay of liver hepatocellular carcinoma (HepG2) cells exposed to different concentrations of QDs. The intracellular uptake and localization of QDs in HepG2 cells, Bacillus subtilis (B. subtilis) and chlamydomonas reinhardtii (C. reinhardtii) were monitored by fluorescence imaging using confocal laser scanning microscopy (CLSM) up to eight hours. Results showed that silica coating yielded final particles{u2019} size around 30 nm possessing strong luminescence property
UR  - http://172.23.153.220/th.pdf
ER  -