Comparative pathological and immunohistochemical studies on natural and experimental infection of velogenic newcastle disease virus in different ages of broiler chickens /
دراســـات باثولوجيه و هستوكيميائية مناعية مقارنة على العدوى الطبيعية و التجريبية لفيروس مرض النيوكاسل الضارى فى الاعمار المختلفة للدجاج اللاحم
Mohamed Refat Mousa ; Supervised Kawkab Abdelaziz Ahmed , Faten Fathy Mohammed , Ayman Hany Eldeeb
- Cairo : Mohamed Refat Mousa , 2019
- 194 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Clinical Pathology
Newcastle disease (ND) remains a constant threat to the poultry industry in Egypt despite intensive vaccination programs. Therefore continuous monitoring of illness in Egypt is essential to detect the new mutant strain of NDV with related pathological pictures as well as identifying the pathogenesis of the isolated new strain is crucial. The present work is divided into two main studies .Firstly; a surveillance study was conducted to investigate the frequency of Newcastle disease virus (NDV) in broiler breeds in different Egyptian governorates between 2016 and 2018. Out of 40 rRT PCR examined tracheal samples, 18 samples were positive for NDV. Phylogenetic analysis of selected 6 positive samples revealed that the isolated strains were belonging to genotype VII. Various clinical and pathological pictures were detected. The clinical signs including depression, greenish diarrhea and neurologic signs, while the pathological alterations were detected in different organ systems including hemorrhagic tracheitis and proventriculitis, lungs congestion, swollen spleen with scattered necrotic foci, hemorrhages on cecal tonsils and atrophied bursa as well as brain perivascular cuffing with diffuse gliosis and nephritis. Immunological detection of NDV antigen clarified the wide distribution of the virus in almost all examined tissues. The second study was an experimental study that was conducted on commercial broiler chickens infected by velogenic Newcastle disease virus (vNDV) at different ages. One hundred and twenty five broiler chickens, non-vaccinated against NDV were allocated into five groups (25 birds each); one group served as control non infected group and four infected groups that were inoculated with 10⁶ EID₅₀ of NDV at 10, 15, 20 and 30 day of age. Quantitative real time RT-PCR for virus re-isolation as well as expression of IL-6 and IFN-Þ genes estimation were performed on lung tissue collected at 3 dpi and kidney tissue at 7 dpi