TY  - BOOK
AU  - Shaymaa Mohammed Abdelrahman Elasmer 
AU  - Hanan Helmy Mohammed Elgendy , 
AU  - Manal Mohamed Kamal , 
AU  - Radwa Marawan Abdelhalim , 
TI  - Evaluation of the genetic polymorphism in pre-microRNA-146a (rs2910164) and level of serum tumor necrosis factor alpha in patients with ischemic stroke and their effects on disease prognosis / 
PY  - 2019///
CY  - Cairo : 
PB  - Shaymaa Mohammed Abdelrahman Elasmer , 
KW  - Ischemic stroke
KW  - Pre-miR-146a
KW  - Rs2910164
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology; Issued also as CD
N2  - Background: Inflammation plays a role in the pathogenesis of atherosclerosis and consequently ischemic stroke. Tumor necrosis factor-Ü (TNF-Ü) initiates a cascade of release of cytokines that increases the expression of adhesion molecules on the vascular endothelium. Moreover, single nucleotide polymorphism in Pre-miR-146a may contribute to susceptibility to ischemic stroke by altering mature miR-146a expression levels. Aim of the work: To investigate the association between the pre-miR-146a C> G (r2910164) polymorphism and serum TNF-Ü among group with ischemic stroke and in a group of unrelated volunteers. Subjects &Methods: This was a case control study on 75 cases with ischemic stroke and 75 sex matched control subjects with age range from 57-65 years. Demographic data and computerized tomography confirmation of ischemic stroke were taken from patients files. All samples were subjected to genomic DNA analysis for pre-miR-146a by real time PCR based method and TNF-Ü measurement by enzyme linked immunosobant assay. Results: The frequency of GG genotype within pre-miR-146a (rs2910164) in ischemic stroke cases was significantly different compared to the control subjects with P = 0.017, OR =3.07, 95%CI (1.2-7.7). A highly statistically significant increase in TNF-Ü level in ischemic stroke subjects when compared to the control group; P <0.001
ER  -