Amira Ahmed Ghonemy Seoudi <br/><br/>
Microbiological studies on clostridium species /  
 دراسات ميكروبيولوجية على انواع الكلوستريديوم 
Amira Ahmed Ghonemy Seoudi ; Supervised Kamelia Osman , Mahmoud Elhariri 
 - Cairo :   Amira Ahmed Ghonemy Seoudi ,   2020 
 - 110 P. :   charts , facsimiles ;   25cm 
<br/><br/>Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology  
<br/><br/> A total of 300 samples representing fecal samples from cows & buffloes showing enteric disorder symptoms suspected to be due to enterotoximeia were analyzed by conventional methods and polymerase chain reaction (PCR). C. perfringens was isolated at the rate of 13.3% from diseased animals, and its presence was confirmed by cultural & biochemical characterization. Multiplex PCR was performed to toxinotype of the 40 toxigenic isolates, and the result showed that all isolates were positive for the alpha toxin gene. Multiplex PCR was performed to phospholipase gene (plc) 28 sample were positive A three selected isolates were identified by sequence analysis of (plc) gene sequencing 
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<br/><br/><label>Subjects--Index Terms: </label>C. perfringens  Fecal samples  Phospholipase gene C