Therapeutic efficacy of osthole on dinitrobenze sulphonic acid induced-colitis in rats /
الكفاءه العلاجيه للاوزول على التهاب القولون المستحث بدينيتروبنزين حمض السلفونيك فى الجرذان
Hanan Khairy Abouarab ; Supervised Mohamed Asem Said Marie , Abeer Mahamoud Badr , Hanan Mohamed Ebead Saleh
- Cairo : Hanan Khairy Abouarab , 2019
- 110 P. : charts , facsmailies; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology
Several mediators were associated with the pathogenesis of inflammatory bowel disease such as oxidative stress through the production of reactive oxygen metabolites, neutrophils infiltration and release of pro-inflammatory cytokines. This study was designed to investigate the therapeutic efficacy of osthole against dinitrobenzene sulfonic acid (DNBS) induced-colitis in rats through its anti- oxidant and anti-inflammatory properties. Colitis was induced in rats by single intracolonic instillation of (250 ol DNBS-25 mg/rat). Then 4 days later, rats were received oral administration of either (osthole 50 mg/kg), (sulfasalazine 500 mg/kg) or both in combination for 7 consecutive days. Body weight, some hematological parameters, colonic malondialdehyde (MDA) and myeloperoxidase activity (MPO), antioxidant parameters, colon injury and mucosa architectures were assessed. T helper (Th1)-related cytokines [Tumor necrosis factor alpha (TNF-Ü) and interferon-gamma (INF-Þ)], Th2-relarted cytokines (interleukin-4 [IL- 4 and IL-10], and Th-17 related cytokines [IL-17] were determined by ELISA. Osthole significantly improved the loss in body weight. That was accompanied with a remarkable amelioration of the disruption of the colonic architecture as well as a significant improvement in the antioxidant defense system. A reduction in MPO and MDA was observed in flamed colon