TY  - BOOK
AU  - Hanan Khairy Abouarab
AU  - Abeer Mahamoud Badr , 
AU  - Hanan Mohamed Ebead Saleh , 
AU  - Mohamed Asem Said Marie , 
TI  - Therapeutic efficacy of osthole on dinitrobenze sulphonic acid induced-colitis in rats / 
PY  - 2019///
CY  - Cairo : 
PB  - Hanan Khairy Abouarab , 
KW  - Osthole Colitis DNBS Antioxidants
KW  - Sulfasalazine Cytokines
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology; Issued also as CD
N2  - Several mediators were associated with the pathogenesis of inflammatory  bowel disease such as oxidative stress through the production of reactive oxygen  metabolites, neutrophils infiltration and release of pro-inflammatory cytokines.  This study was designed to investigate the therapeutic efficacy of osthole against  dinitrobenzene sulfonic acid (DNBS) induced-colitis in rats through its anti- oxidant and anti-inflammatory properties. Colitis was induced in rats by single  intracolonic instillation of (250 ol DNBS-25 mg/rat). Then 4 days later, rats were  received oral administration of either (osthole 50 mg/kg), (sulfasalazine 500  mg/kg) or both in combination for 7 consecutive days. Body weight, some  hematological parameters, colonic malondialdehyde (MDA) and myeloperoxidase  activity (MPO), antioxidant parameters, colon injury and mucosa architectures  were assessed. T helper (Th1)-related cytokines [Tumor necrosis factor alpha  (TNF-Ü) and interferon-gamma (INF-Þ)], Th2-relarted cytokines (interleukin-4 [IL- 4 and IL-10], and Th-17 related cytokines [IL-17] were determined by ELISA.  Osthole significantly improved the loss in body weight. That was accompanied  with a remarkable amelioration of the disruption of the colonic architecture as well  as a significant improvement in the antioxidant defense system. A reduction in  MPO and MDA was observed in flamed colon
ER  -