Advanced studies on salmonellosis in sheep and goats /
دراسات متقدمة عن مرض السالمونيلا فى الأغنام والماعز
Manar Magdy Farouk Sayed Abdelshafy ; Supervised Amal Abdelaziz Elmolla , Fayez Awad Allah Salib , Yousef Adel Soliman
- Cairo : Manar Magdy Farouk Sayed Abdelshafy , 2021
- 209 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Internal Medicine and Infectious Diseases
Continuous emergence of antimicrobial resistant (AMR) salmonellosis poses a threat for treatment of infectious diseases in animals and humans. Identification of factors associated with development of AMR salmonellosis and providing alternative antimicrobials to limit this threat are of a great value. Therefore, the present study aimed to estimate the prevalence of salmonellosis in diarrheic sheep and goats belonging to Giza governorate in Egypt, characterize phenotypic antimicrobial sensitivity pattern of the isolated Salmonella spp. strains, and identify the management factors associated with perpetuation of Salmonella spp pathogen among the investigated flocks. Also, the study aimed to investigate the presence of enterotoxin (stn) gene in the isolated strains and its role in development of diarrhea, and to analyze the genetic relatedness between the isolated strains and other strains retrieved from GenBank based on partial codon sequence of stn gene in order to provide a scientific basis for the implementation of practical preventive measures. Furthermore, this study aimed to investigate the ability of silver nanoparticles, Bulgarian propolis ethanolic extract and locally prepared inactivated ISA-70 mantonide adjuvanted S. Enteritidis vaccine to alternate antimicrobials to control the development of AMR problem. A cross sectional (census) survey study was conducted, and a total number of 518 diarrheic sheep and goats belonging to 7 mixed flocks of sheep and goats were enrolled from which, rectal swabs were collected and subjected to culture for Salmonella spp. The presumptive identified Salmonella colonies were biochemically identified, serotyped and molecularly confirmed to be Salmonella spp. by amplification of invA gene using polymerase chain reaction (PCR) technique. The confirmed Salmonella spp. strains were examined for their susceptibility to 10 of the most commonly used antimicrobials using minimum inhibitory concentration (MIC) and to 11 antimicrobials using disc-diffusion method