Studies on virulence and antimicrobial resistance of Mycoplasma species recovered from camel /
دراسات على ضراوة ومقاومة المكوبلازما المعزولة من الجمال للمضادات الحوة
Walaa Mohammed Abdelazeem Mohammed ; Supervised Kamelia Mahmoud Osman , Ahmed Oraby Hassan , Zeinab Roshdy Mohammed
- Cairo : Walaa Mohammed Abdelazeem Mohammed , 2021
- 168 P. : charts ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
This study was carried out on 460 samples obtained from apparently healthy and diseased Camels. 26.3 % of samples were positive to the primary isolation of Mollicutes. Recovery rate of Mycoplasma was 13.2% from diseased slaughtered Camels. Biochemical and molecular identification (PCR) of Mycoplasma isolates showed that 21.31 % were M. bovis and 78.68 % were M. arginine. On performing phenotypic characterization of isolates, it was found that M. bovis isolates showed high resistance to ciprofloxacin and erythromycin in percentage of 100% & 76.9% respectively, while M. arginini showed high resistance to ciprofloxacin, erythromycin, doxycycline and lincomycin in percentage 83.3%, 79.2%, 64.6% and 58.3% respectively. 20% of M. bovis and 19% of M. arginini isolates showed catalase activity. 100% M. bovis and M. arginini isolates have haemolytic activity and H2S producers. M. bovis and M. arginini isolates were found to have poor adhesion and biofilm formation abilities. The par C, gyr A ,vsp A, uvr C and gap A genes were not detected in M. arginini .While par C and vsp A were detected in 15% of M. bovis isolates