Analytical studies of some drugs containing amide group and its mono-acyl derivative /
دراسات تحليلية لبعض الادويه التى تحتوى على مجموعه الاميد ومشتقها احادى الاسيل
Aya Hosam Helmy ; Supervised Badr A. Elzeiny , Soheir A. Ahmed , Nadia F. Youssef
- Cairo : Aya Hosam Helmy , 2021
- 163 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Analytical Chemistry
The health and the safety of pharmaceutical products consumers are protected by the national control laboratories. Prior the pharmaceutical product is approved to the market several tests must be applied to ensure that these products meet the accepted standard of quality, efficacy and safety. However, time consuming method is usually stood as barrier to achieve fast results. Moreover, developing country laboratories are suffering from deficiency of sophisticated analytical instruments. This study concerned with developing fast, simple, inexpensive and accurate accessible analytical methods using chromatographic and spectroscopic techniques for the simultaneous determination of selected drugs containing amide group like perampanel and carbamazepine or its mono-acyl derivative like aniracetam. Stability-indicating chromatographic methods were applied to determine aniracetam in presence of its degradation products using 25 cm, C18 column with limit of detection of 0.216 æg/ml for HPLC method, 0.009 æg/band for NP-TLC and 0.008 for RP-TLC methods. Comparative kinetics studies and pH rate-profile were applied to determine the rate and order of degradation reaction using the developed HPLC-UV method. Spectroflourimetric method was used to determine aniracetam by fluorescence quenching of tyrosine and L-tryptophan fluorescent amino acids with LOD 0.104æg/ml and 0.140æg/ml, respectively