TY  - BOOK
AU  - Sarah Hamdy Ahmed Mohamed
AU  - Mohamed Ahmed Badawy , 
AU  - Mohamed Elsayed Elshinawi , 
AU  - Sherif Abdelaziz Ibrahim , 
TI  - Extracellular vesicles as potential markers for inflammatory breast cancer / 
PY  - 2021///
CY  - Cairo : 
PB  - Sarah Hamdy Ahmed Mohamed , 
KW  - MicroRNAs
KW  - Small Extracellular Vesicles (sEVs)
KW  - Sxosomes
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology; Issued also as CD
N2  - Inflammatory breast cancer (IBC) is a rare, but aggressive entity of breast carcinoma  associated with rapid dermal lymphatic invasion in young females. It is either poorly or  misdiagnosed as mastitis due to the absence of a distinct lump. Small extracellular vesicles  (sEVs) (20-200nm) circulating in liquid biopsies are a novel class of minimally invasive  diagnostic alternatives to invasive tissue biopsies. In the cancer microenvironment, they  modulate cancer progression via shuttling their encapsulated cargo including microRNAs  (miRNAs) into recipient cells to either trigger signaling or induce malignant transformation of  targeted cells. While small non-coding regulatory miRNAs are already known cancer  biomarkers, exosomal miRNAs serve as a novel class of diagnostic biomarkers. The present  study mainly aimed to evaluate the expression levels of sEV- derived miRNAs relevant to IBC  pathogenesis. This prospective case-control study included 77 females; 57 were diagnosed with  breast cancer (34 non-IBC and 23 IBC), while 20 were healthy volunteers. All have signed an  informed consent to be enrolled in this investigation. Patients included were neither pregnant nor  issued with bloodborne or autoimmune disease, while healthy subjects had no oncologic history.  Plasma sEVs less than 200 nm were isolated using modified cost-effective polyethylene glycol  (PEG)-based precipitation method and compared to standard methods, namely  ultracentrifugation, and a commercial kit. The successful isolation was verified by dynamic light  scattering (DLS), transmission electron microscopy (TEM), western blot (WB), and dot blot  (DB)
UR  - http://172.23.153.220/th.pdf
ER  -