Diagnosis of Subclinical Mastitis Using Different Methods, with Isolation of Different Causative Organisms from Cow's Milk /
تشخيص التهاب الضرع الكامن باستخدام طرق مختلفة مع عزل بعض الميكروبات المسببة له فى حليب الابقار
Elshimaa Nageh Kamel Mehany ; Supervised Said Sayed Sallam , Karima Mogahed Fahim , Mohamed Abdelzaher Elshafaie
- Cairo : Elshimaa Nageh Kamel Mehany , 2022
- 124 P . : charts ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Food Hygiene and Control
A total of 214 bulk tank milk (BTM) samples and a total of 832 individual quarter milk samples (IQMS) of clinically recognized and apparently normal quarters of 208 lactating cows were collected from different dairy farms in Alexandria, Menofia and El-Dakahlia Governorates, Egypt. Bulk tank somatic cell count and associated risk factors influencing the hygienic quality and chemical composition of raw milk were investigated. Different mastitis indicators as screening tests (California Mastitis Test "CMT" and Somatic Cell Count "SCC"); milk constituents; pH value; enzymes activities (milk lactate dehydrogenase LDH, Acid phosphatase ACP, alkaline phosphatase ALP and glutathione peroxidase GPx) and bacteriological examination were applied on IQMS to assess the potential relationships between these parameters and their possibility of being markers for diagnosis of mastitis. In BTM analysis, the mean values of SCC/ml, Aerobic Plate Count APC (cfu/ml), Coliform count (MPN/ml), S. aureus count and B. cereus count (cfu/ml) were 6.85105±0.33105, 7.09106±2.76106, 2.77104±0.63104, 1.27103±0.25103 and 3.32103±0.13103, respectively. The results reveal the presence of S. aureus; CNS; St. uberis, E. coli and B. cereus with different percentages from the examined samples. In the level of IQMS, the mean SCC of all positive and negative CMT samples was 8.78105±3.49x104 and 1.63105±6.29x103 cells/ml respectively. The mean values of milk fat, protein, SNF, lactose and ash content were significantly higher in IQMS from normal quarters than those from subclinical or clinical ones; while chlorine %, pH values and enzymes activity increased with the progress of infection. S. aureus, E. coli, coagulase negative staphylococci (CNS), St. uberis and other streptococci could be isolated at varying percentages from the IQMS. Meanwhile, Trueperella pyogenes, St. agalactiae and St. dysgalactiae failed to be isolated from any of the examined IQMS. Consequently, LDH and ACP proved to be better and more reliable biomarkers for early diagnosis of subclinical mastitis