TY  - GEN
AU  - Mohamed Abd El rahman Mohamed Osman
AU  - Essam Amin Nasr , 
AU  - Magdy Ahmed Elsayed Ghoneim , 
AU  - Salah ElDien Abdelkarim Selim , 
TI  - Trials for Vaccine Preparation Against Tuberculosis / 
PY  - 2006///
CY  - Cairo : 
PB  - Mohamed Abdelrahman Mohamed Osman , 
KW  - DNA
KW  - ESAT - 6
KW  - PQE
N1  - Thesis (PH.D.) - Cairo University - Faculty Of Veterinary Medicine - Department Of Microbiology , Bacteriology , Mycology and Immunology; Issued also as CD
N2  - The current study aimed for the cloning and expression of ESAT - 6 protein antigen from Mbovis and its use alone or plus adjuvant as a vaccine against bovine tuberculosisPrimarily , Mbovis genomic DNA was extracted using triazol purified , concentrated and then esat - 6 gene was amplified by PCR and assessed by agarose gel electrophoesisesat - 6 gene was ligated to an expression vector , PQEAfter transformation by electroporation of the expression host Ecoli with the recombinant PQE plasmid , the expression was induced using 10 mM of IPTG , two bands were seen in the SDS - PAGE analysis , the 18 kDa which represented the dimmer forms of their ESAT - 6 antigenThe his - tagged r - ESAT - 6 antigen was then purified by metal affinity chromatography using Ni - NTA agarose
ER  -