Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Vegetable

The aim of this work was to improvement of the performance of strawberry plants in tissue cultures. Also hardened the in vitro plantlets to the open field. To achieve optimal number of shoots per explant and cluster weight (g) cultured shoot tip on MS medium and boxus (1974) medium supplemented with 1.0 mg / l BA and 0.1 mg / l IBA. Sweet charlie cultivar producing more shoot number than florida cultivar, shoot tip explant producing highest values of number of shoot and cluster weight, MS medium and cossio and Menin 1982 medium revealed similar values of number of shoot and cluster weight, the darkness conditions (24h darkness for 15 days then later normal conditions) showed higher values of number of shoots and cluster weight, 3.0 mg/l TDZ producing highest values of number of shoot and cluster weight when meristem explant is used but when the shoot tip explant used 1.0 mg / l BA producing highest values of number of shoot and cluster weight. The third subculture obtained the best values of number of shoot and cluster weight. Sweet Charlie cultivar plantlets obtained maximum values of plantlet height on hormone free MS medium at half or double strength while Florida cultivar on MS medium at half strength supplemented with 1.0 mg / l IBA

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