Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology

In this study the aluminum hydroxide adjuvant gel used widely in a lot of human and animal vaccines and currently solely used as an adjuvant in the locally produced inactivated Rift Valley Fever vaccine was treated by ultrasonication in the presence of trehalose for dispersion of its particles. The dispersed aluminum hydroxide adjuvant was extensively studied where various physical characters were determined. The particle size of aluminum hydroxide was significantly reduced as the aggregates size ranging from 1-20 æm. As a result of dispersion, the TEM image of vaccine showed a change of the content from nonuniform and large aggregates of aluminum hydroxide with Rift Valley fever inactivated virus diffused in it to uniform particles with aluminum hydroxide adsorbed on virus surface and having a diameter of 300 nm. The vaccine with dispersed aluminum hydroxide was used in immunizing mice and showed superiority in immunogenicity when compared to the conventional vaccine

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