Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Veterinary Pharmacology

The present work was carried out to detect the phytochemical properties of Moringa oleifera leaves methanol extract MOLME and its fractions by using preliminary phytochemical and GC-Mass analysis and detect the pharmacological properties as antidiarrheal, antibacterial, antioxidant and hepatoprotective .The MOLME was subjected to fractionation by using five types of solvents with different polarization; n-hexane, dichloromethane, ethyl acetate, n-butanol and water. The phytochemical results showed that MOLME contains many chemical compounds with different concentration and different effects all thus compound were distributed after fractionation and concentrated in different fractions. Pharmacology studies showed significant antidiarrheal effect in MOLME this effect was concentrated in tow fractions n-hexane and dichloromethane. The result also showed that confined antibacterial effect for MOLME concentrated in n-butanol fraction with moderate effect in aqueous fraction. MOLME showed moderate antioxidant activity in vitro against DPPH and high activity for n-butanol and ethyl acetate fraction. In vivo MOLME and n-hexane, dichloromethane and aqueous showed significant effect in several parameters protein profile, liver function and antioxidant biomarker

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