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Production and preservation of virusfree citrus plants by using tissue culture / Nancy Danial Girgis Sorial ; Supervised Amina Hamed Gomaa , Mohamed Helmy Abdelzaher , Adelelsawy Mohamed

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Nancy Danial Girgis Sorial , 2014Description: 219 P. : charts , facsimiles ; 25cmOther title:
  • إنتاج وحفظ نباتات موالح خال{u٠٦أأ}ة من الف{u٠٦أأ}روس بستخدام زراعة الأنسجة [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Pomology Summary: This study was carried out in Plant Biotechnology Department, National Research Center during the period from 2008 to 2013. Somatic embryogenesis from stigma and style explants of infected and uninfected trees with CPsV from different citrus genotypes showed significant effects on embryogenesis potentials on the same medium (MS + 3 mg/l BAP + 500 mg/l ME + 50 g/l sucrose) . Results indicated that stigma explants was better for callus and SE induction whereas, style explants produced callus only. Uninfected Washington navel orange produced the highest callus and SE formation percentages compared with infected ones or Valencia orange and local mandarin. Stigmaderived plantlets were CPsV-free as tested by RT-PCR and true to type as tested by RAPD-PCR. All virus-free microshoots preserved on media with 0.1 M sorbitol, sugar-free, GR-free and sugar & GR-free medium for 4 months able to proliferate new shoots
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.13.Ph.D.2014.Na.P (Browse shelf(Opens below)) Not for loan 01010110064235000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.13.Ph.D.2014.Na.P (Browse shelf(Opens below)) 64235.CD Not for loan 01020110064235000

Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Pomology

This study was carried out in Plant Biotechnology Department, National Research Center during the period from 2008 to 2013. Somatic embryogenesis from stigma and style explants of infected and uninfected trees with CPsV from different citrus genotypes showed significant effects on embryogenesis potentials on the same medium (MS + 3 mg/l BAP + 500 mg/l ME + 50 g/l sucrose) . Results indicated that stigma explants was better for callus and SE induction whereas, style explants produced callus only. Uninfected Washington navel orange produced the highest callus and SE formation percentages compared with infected ones or Valencia orange and local mandarin. Stigmaderived plantlets were CPsV-free as tested by RT-PCR and true to type as tested by RAPD-PCR. All virus-free microshoots preserved on media with 0.1 M sorbitol, sugar-free, GR-free and sugar & GR-free medium for 4 months able to proliferate new shoots

Issued also as CD

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