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Detection of clostridium perfringens and staphylococcus aureus in fish and seafood / Walaa Salah Abdelbaki Omar Hammouda ; Supervised Nashwa Abdslsalam Ezz Eldeen , Mahmoud Dardiri Mohammed Elhariri , Ahmed Mohammed Ammar

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Walaa Salah Abdelbaki Omar Hammouda , 2016Description: 144 P. : photographs ; 25cmOther title:
  • الكشف عن الكوليستريديم بيرفرنجينز و الأستافيلوكوكاس أوريس فى السمك و المأكولات البحرية [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology Summary: Out of 150 fish and seafood samples twenty samples were identified as clostridium perfringens type "A" (alpha toxin) using multiplex polymerase chain reaction (MPCR) and detected cpe gene with percentage of 15% and 20% using uniplex PCR and SYBR green real time PCR, respectively. Staphylococcus aureus coagulase gene was detected in 12 fish and seafood samples using uniplex PCR, detection of staphylococcus aureus enterotoxins B (seb) gene in one sample and Staphylococcus aureus enterotoxins C (sec) gene in other two samples using MPCR. In addition, detection of staphylococcus aureus enterotoxins A (sea) gene and staphylococcus aureus enterotoxins D (sed) gene in another two sample using SYBR Green real time PCR from thirteen examined fish and seafood samples. SYBR green real time PCR more sensitive, qualitative and quantitative to rapidly detection clostridium perfringens and staphylococcus aureus their toxins genes directly from fish and seafood
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.Ph.D.2016.Wa.D (Browse shelf(Opens below)) Not for loan 01010110070754000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.Ph.D.2016.Wa.D (Browse shelf(Opens below)) 70754.CD Not for loan 01020110070754000

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Out of 150 fish and seafood samples twenty samples were identified as clostridium perfringens type "A" (alpha toxin) using multiplex polymerase chain reaction (MPCR) and detected cpe gene with percentage of 15% and 20% using uniplex PCR and SYBR green real time PCR, respectively. Staphylococcus aureus coagulase gene was detected in 12 fish and seafood samples using uniplex PCR, detection of staphylococcus aureus enterotoxins B (seb) gene in one sample and Staphylococcus aureus enterotoxins C (sec) gene in other two samples using MPCR. In addition, detection of staphylococcus aureus enterotoxins A (sea) gene and staphylococcus aureus enterotoxins D (sed) gene in another two sample using SYBR Green real time PCR from thirteen examined fish and seafood samples. SYBR green real time PCR more sensitive, qualitative and quantitative to rapidly detection clostridium perfringens and staphylococcus aureus their toxins genes directly from fish and seafood

Issued also as CD

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