Assessment of the diagnostic potential of some genetic markers of diabetes mellitus in the onset of diabetic nephropathy in type 2 diabetic patients / Demiana Badry Helmy Hanna ; Supervised Mervat Elsayed Mohmed Abdelkader , Ahmed Osman Egiza

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Demiana Badry Helmy Hanna

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TextLanguage: English Publication details: Cairo : Demiana Badry Helmy Hanna , 2015Description: 164 P. : charts ; 25cmOther title:

تقييم القدرة التشخيصية لبعض الدلالات الوراثية لمرض البول السكرى فى حالة ظهور الاعتلال الكلوى فى مرضى السكر من النوع الثانى [Added title page title]

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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry Summary: Monocyte Chemoattractant Protein-1 (MCP-1) and Osteopontin (OPN) are the most important macrophage chemokines that characterize with increasing their expression in type 2 diabetic nephropathy, so measuring serum concentration of OPN and relative quantification (RQ) of mRNA levels of OPN and MCP-1 is of great significance in the diagnosis and intervention of early diabetic nephropathy. The aim of the present study was designed to find sensitive biomarkers for diagnosis and intervention of early phase of diabetic nephropathy by determining the levels of different biomarkers (MCP-1 and OPN) at different stages of diabetic nephropathy and to see their correlations with other risk factors of diabetic nephropathy in Egyptian type2 diabetic subjects. Mean levels of the two biomarkers OPN (serum concentration and RQ of mRNA levels) and MCP-1(RQ of mRNA levels) in normoalbuminuric patients with type 2 diabetes were significantly higher than in control subjects (p<0.0001). Compared with the normoalbuminuric group, the mean levels of the two biomarkers in the microalbuminuric and macroalbuminuric groups were significantly higher (p<0.0001). Also, the means level of the two biomarkers in the macroalbuminuric group were significantly higher than those in the microalbuminuric group (P<0.0001). OPN (serum concentration and RQ of mRNA levels) and MCP-1(RQ of mRNA levels) were strongly correlated positively with microalbumin concentration and negatively with eGFR levels. Receiver operating curve (ROC) analysis showed that ROC curve for MCP-1 (RQ of mRNA levels) was higher sensitivity and specify (97.50 % and 98.00%, respectively) than ROC curve for OPN serum concentration (87.50% and 92.00%) and ROC curve for RQ mRNA levels (80.00% and 96.00)

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Item type	Current library	Home library	Call number	Copy number	Status	Date due	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.12.02.Ph.D.2015.De.A (Browse shelf(Opens below))		Not for loan		01010110069225000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.12.02.Ph.D.2015.De.A (Browse shelf(Opens below))	69225.CD	Not for loan		01020110069225000

Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry

Monocyte Chemoattractant Protein-1 (MCP-1) and Osteopontin (OPN) are the most important macrophage chemokines that characterize with increasing their expression in type 2 diabetic nephropathy, so measuring serum concentration of OPN and relative quantification (RQ) of mRNA levels of OPN and MCP-1 is of great significance in the diagnosis and intervention of early diabetic nephropathy. The aim of the present study was designed to find sensitive biomarkers for diagnosis and intervention of early phase of diabetic nephropathy by determining the levels of different biomarkers (MCP-1 and OPN) at different stages of diabetic nephropathy and to see their correlations with other risk factors of diabetic nephropathy in Egyptian type2 diabetic subjects. Mean levels of the two biomarkers OPN (serum concentration and RQ of mRNA levels) and MCP-1(RQ of mRNA levels) in normoalbuminuric patients with type 2 diabetes were significantly higher than in control subjects (p<0.0001). Compared with the normoalbuminuric group, the mean levels of the two biomarkers in the microalbuminuric and macroalbuminuric groups were significantly higher (p<0.0001). Also, the means level of the two biomarkers in the macroalbuminuric group were significantly higher than those in the microalbuminuric group (P<0.0001). OPN (serum concentration and RQ of mRNA levels) and MCP-1(RQ of mRNA levels) were strongly correlated positively with microalbumin concentration and negatively with eGFR levels. Receiver operating curve (ROC) analysis showed that ROC curve for MCP-1 (RQ of mRNA levels) was higher sensitivity and specify (97.50 % and 98.00%, respectively) than ROC curve for OPN serum concentration (87.50% and 92.00%) and ROC curve for RQ mRNA levels (80.00% and 96.00)

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