Physiological and molecular changes contributed to salt stress response in some grapevine rootstocks / Mohamed Alaa Mohamed Abdelfattah ; Supervised Ahmed Tawfik Mohamed Salem , Mohamed Abdelaziz Abdelmohsen , Mohamed Abdelsalam Rashed

Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Pomology

A pot experiment was carried out during two seasons of 2016 and 2017 under shadehouse conditions.Four salinity treatments (control, 1500, 2500 and 3500 ppm NaCl) were used to investigate the effect of irrigation with saline water on growth, morphological and physiological parameters in grapevine rootstock(Dogridge, Freedom, Paulsen 1103, Ramsey, Ruggeri 140 and SO₄). Transcriptome and expression analysis was performed on the most tolerant rootstocks (Ruggeri 140 and Ramsey) treated with 3500 ppm NaCl. Salt injury symptoms appeared in Dogridge, Freedom, Paulsen 1103 and SO₄ rootstocks under all salinity levels. Generally, survival percentage was decreased with increasing salinity level in irrigation water. Growth and morphological parameters were significantly decreased by irrigation with saline water in Dogridge, Freedom, Paulsen 1103, and SO₄. In contrast, growth of Ramsey and Ruggeri 140 was stimulated under low salinity level (1500 ppm).Toxicity of sodium and chloride severely affected Dogridge, Freedom,Paulsen 1103 and SO₄ rootstocks as they accumulated high amount of both elements than Ramsey and Ruggeri 140. Leaf proline content was increased gradually in all rootstocks with increasing salinity level in irrigation water. Ramsey and Ruggeri 140 rootstocks showed a lower decrease in chlorophyll pigments than other rootstocks. Reduction in stomatal conductance with an associated decrease in transpiration rate was observed in all rootstocks with increasing salinity level in irrigation water

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