Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry

In the last decades, the perception of non-coding RNAs (ncRNAs) was changed from being as junk transcriptional products to acting as a functional epigenetic regulator of genes due to their crucial roles in regulating various biological and pathological processes. ncRNAs encompassed major classes like the well-known microRNAs (miRNAs) and the most recently acknowledged long non- coding RNAs (lncRNAs). Previous studies have revealed a complicated interplay between many miRNAs and LncRNAs. This miRNA-LncRNA crosstalk was involved in a great number of human diseases such as Hepatitis infection. Circulating microRNAs have a vital role in Hepatitis B virus (HBV) diagnosis and therapeutics. miR-100 was reported to be associated with various aspects of HBV biology. This study focused on microRNA-100 (miR-100) and Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) Single Nucleotide Polymorphisms (SNPs) and their contribution to an individual's susceptibility and prognosis of HBV infection correlated to their expression level. The effect of these SNPs on their expression levels was also evaluated on 200 subjects divided into 100 HBV infected patients versus 100 age-and-sex- matched healthy individuals served as a control group. SNP detection was performed using polymerase chain reaction sequence-specific primer technique (PCR-SSP) for miR-100 SNP rs1834306 T/C while for MALAT1 SNP rs619586A/G was detected using restriction fragment length polymorphism-PCR (RFLP-PCR) techniques. miR-100 and MALAT1 expressions were measured by quantitative real-time PCR (qRT-PCR) Egyptian population

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