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Mining Red Sea microbiota for antimicrobial activity and their potential application in nanoparticles-based combinations / Hala Salah Eldin Helal Mahmoud ; Supervised Hamdallah Hafez Zedan , Amr Saeed Hanoura , Hayam Mahmoud Hussein

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Hala Salah Eldin Helal Mahmoud , 2018Description: 110 P. : charts, facsimiles ; 25cmOther title:
  • استخدام الكائنات الدقيقه العزولة من البحر الاحمر لانتاج المضادات الحيويه وامكانيه تعزيز نشاطها بالدمج مع جسيمات النانو [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology Summary: Objective: The objective of this work was to isolate bacteria from Red Sea invertebrates, determine the antimicrobial activity of the bacterial extract, and screen for the biosynthetic gene clusters (PKS and NRPS) which could be involved in the production of bioactive secondary metabolites. Then, combining the bacterial extract with the antimicrobial polysaccharide chitosan nanoparticles and testing if the antimicrobial activity will be enhanced.Methods: Eleven different samples of Red Sea invertebrates were collected in Egyptian region and the associated microorganisms were cultivated and screened for the antimicrobial activity against five reference strains (Gram positive Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 9144, Gram-negative E.coli ATCC 10536 and Pseudomonas aeruginosa ATCC25619, and Candida albicans ATCC 90028).Then these isolates were screened for the presence of the biosynthetic genes PKS I&II and NRPS I&II. After that, the genes were cloned using (TOPO TA kit) and sequenced using Applied Biosystem. Finally, the structures of the secondary metabolite gene clusters in the genomes of isolate HHF-8 were predicted by The Natural Product Domain Seeker (NaPDoS) software.The second antimicrobial agent, chitosan nanoparticles were synthesized and combined with Bacillus amyloliquefaciens's extract and characterized using Nanotrac wave II for particle size measurement. Finally, the antimicrobial activity was tested for the chitosan, the most potent isolate's extract and chitosan-extract nanocomposite, against the five reference strains (Gram positive Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 9144, Gram-negative E.coli ATCC 10536 and Pseudomonas aeruginosa ATCC25619, and Candida albicans ATCC 90028)
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.08.06.Ph.D.2018.Ha.M (Browse shelf(Opens below)) Not for loan 01010110077751000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.08.06.Ph.D.2018.Ha.M (Browse shelf(Opens below)) 77751.CD Not for loan 01020110077751000

Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology

Objective: The objective of this work was to isolate bacteria from Red Sea invertebrates, determine the antimicrobial activity of the bacterial extract, and screen for the biosynthetic gene clusters (PKS and NRPS) which could be involved in the production of bioactive secondary metabolites. Then, combining the bacterial extract with the antimicrobial polysaccharide chitosan nanoparticles and testing if the antimicrobial activity will be enhanced.Methods: Eleven different samples of Red Sea invertebrates were collected in Egyptian region and the associated microorganisms were cultivated and screened for the antimicrobial activity against five reference strains (Gram positive Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 9144, Gram-negative E.coli ATCC 10536 and Pseudomonas aeruginosa ATCC25619, and Candida albicans ATCC 90028).Then these isolates were screened for the presence of the biosynthetic genes PKS I&II and NRPS I&II. After that, the genes were cloned using (TOPO TA kit) and sequenced using Applied Biosystem. Finally, the structures of the secondary metabolite gene clusters in the genomes of isolate HHF-8 were predicted by The Natural Product Domain Seeker (NaPDoS) software.The second antimicrobial agent, chitosan nanoparticles were synthesized and combined with Bacillus amyloliquefaciens's extract and characterized using Nanotrac wave II for particle size measurement. Finally, the antimicrobial activity was tested for the chitosan, the most potent isolate's extract and chitosan-extract nanocomposite, against the five reference strains (Gram positive Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 9144, Gram-negative E.coli ATCC 10536 and Pseudomonas aeruginosa ATCC25619, and Candida albicans ATCC 90028)

Issued also as CD

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