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The vaccines and their potential impact on antimicrobial resistance / Basma Mohamed Hamed Ahmed ; Supervised Mona Ibrahem Elenbaawy , Eman Ragab Mohamed Mostafa

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Basma Mohamed Hamed Ahmed , 2022Description: 99 P. : charts , facsimiles ; 25cmOther title:
  • الفاكسينات و تأثيرها المحتمل على المقاومة للمضادات الحيوية [Added title page title]
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Dissertation note: Thesis (M.Sc. ) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology Summary: Antimicrobial resistance (AMR) is now recognized as a major threat to global health. The present work aims to study the role of vaccine against AMR in broiler chickens using live avirulent multi-drug resistant E. coli vaccine also, investigate the additive effect of chitosan nanoparticles either as encapsulated or loaded form in another two live inactivated E. coli vaccine. Different samples from internal organs were collected from broiler from 10-38 day old. APEC was isolated by conventional culture methods and VITEK@ 2 COMPAT (bioMérieux, France) with prevalence ratio 67.5 %. Genes associated with virulence (iutA, fimC, and papC) were identified by PCR with the prevalence of 84.4%, 74%, and 54.8% of cases, respectively. Concerning antibiotic sensitivity test among the most resistant drugs, the highest rate of resistance was against Ampicillin (AM) (100%), Trimethoprim-Sulfamethoxazole (80%) Ampicillin-sulbactam (AMS) (78.5), Ceftazidime (CAZ) 75.5%, cefepime (CPM) 74 %. The vaccinal strain was selected to be avirulent and multidrug resistant. Four groups were designed for E. coli experimental vaccinal trial. The first was a negative control, the second was chitosan capsulated-antigen, the third was chitosan loaded-Ag, and the last group was immunized with live avirulent strain. Experiment vaccination revealed that antibody titer was measured by indirect ELISA and confirmed results by MAT shows that Only group 2 and 3 Significantly produced the highest response among groups. Also, the recovery of APEC from internal organs was the lowest in group 2,3 in comparison with group 4. It could be concluded that, administration of E. coli killed vaccine either loaded or encapsulated with chitosan nanoparticles promoted humeral immune response and antibody production as well as reduced APEC challenge loads. Also, E. coli vaccines based on chitosan are therefore a viable alternative to current vaccines for poultry
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.M.Sc.2022.Ba.V (Browse shelf(Opens below)) Not for loan 01010110085519000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.M.Sc.2022.Ba.V (Browse shelf(Opens below)) 85519.CD Not for loan 01020110085519000

Thesis (M.Sc. ) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Antimicrobial resistance (AMR) is now recognized as a major threat to global health. The present work aims to study the role of vaccine against AMR in broiler chickens using live avirulent multi-drug resistant E. coli vaccine also, investigate the additive effect of chitosan nanoparticles either as encapsulated or loaded form in another two live inactivated E. coli vaccine. Different samples from internal organs were collected from broiler from 10-38 day old. APEC was isolated by conventional culture methods and VITEK@ 2 COMPAT (bioMérieux, France) with prevalence ratio 67.5 %. Genes associated with virulence (iutA, fimC, and papC) were identified by PCR with the prevalence of 84.4%, 74%, and 54.8% of cases, respectively. Concerning antibiotic sensitivity test among the most resistant drugs, the highest rate of resistance was against Ampicillin (AM) (100%), Trimethoprim-Sulfamethoxazole (80%) Ampicillin-sulbactam (AMS) (78.5), Ceftazidime (CAZ) 75.5%, cefepime (CPM) 74 %. The vaccinal strain was selected to be avirulent and multidrug resistant. Four groups were designed for E. coli experimental vaccinal trial. The first was a negative control, the second was chitosan capsulated-antigen, the third was chitosan loaded-Ag, and the last group was immunized with live avirulent strain. Experiment vaccination revealed that antibody titer was measured by indirect ELISA and confirmed results by MAT shows that Only group 2 and 3 Significantly produced the highest response among groups. Also, the recovery of APEC from internal organs was the lowest in group 2,3 in comparison with group 4. It could be concluded that, administration of E. coli killed vaccine either loaded or encapsulated with chitosan nanoparticles promoted humeral immune response and antibody production as well as reduced APEC challenge loads. Also, E. coli vaccines based on chitosan are therefore a viable alternative to current vaccines for poultry

Issued also as CD

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