Studies on Staphylococcus aureus polyvalent vaccine to control mastitis among dairy cows in Egypt / Ashraf Megahed Abdelmaksoud Elsheikh Eissa ; Supervised Diea Gamal Eldin Aboelhassan Elithy , Mohamed Abdelmonaem Salem , Ahmed Samir Mohamed

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Internal Medicine and Infectious Diseases

Staphylococcus aureus is a common udder pathogen in dairy cows, and cause severe mastitis problems in some herds in Egypt. In herds where normal control measures are not successful, vaccination might be an additional tool to use if sufficiently efficient. In the last years the knowledge on cow mastitis is remarkably improving, nevertheless the attention has been never focused on vaccination as preventive strategy for the control of mastitis. A successful Staphylococcus aureus vaccine should elicit a long-term antibody response that prevents establishment of the infection. As sera of immunized pregnant heifers with S. aureus lysed cells formulated with a classical adjuvant stimulate antibodies production that inhibited internalization in mammary epithelial cells (MEC) and increased phagocytosis by milk macrophages, the aim of the present study was to evaluate the humoral immunological response to a commercially available vaccine (Lysigin{u2122}, Boehringer Ingelheim Vetmedica Inc., St. Joseph, MO) and its potential use as a preventive way for S. aureus mastitis in Holstein cows Vaccinated (V) and not vaccinated (N-V) groups, of 60 cows, were selected from one herd of the same farm. The herd received a double vaccination (Lysigin{u2122} ), 30 and 15 days before calving, and 5 months after calving)

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