Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Background: Acute myeloid leukemia (AML) is a broad range of disorders that are all characterized by an arrest of maturation along with uncontrollable proliferation of hematopoietic progenitor cells, MIF was highly expressed in tumor tissues and it is involved self-sufficient proliferation, insensitivity to anti-proliferative signals, evasion of apoptosis, the potential for unlimited replication, the maintenance of angiogenesis, and for tissue invasion and metastasis. Aim of work: The aim of the present study was to determine the potential association between MIF 173 G/C polymorphism and acute myeloid leukemia and to determine whether its presence has a role as a risk factor for the disease. Subjects & Methods: The study was conducted on 50 de novo AML patients and 100 age and sex matched healthy adults. Polymerase chain reaction Restriction Fragment Length Polymorphism (PCR-RFLP) technique was performed for the detection of MIF 173 G/C polymorphism. Results: MIF mutant genotypes (GC and CC), allele (C), were lower among AML patients compared to controls, with no statistical significance (P value = 0.563). There was a statistically significant difference between MIF mutant genotypes and some FAB subtypes namely: M0+M6+M7 (p=0.039) as well as higher total leukocytic counts (p=0.002), lower platelet level (p=0.022) and higher peripheral blood blasts percent (p=0.010)

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