Genetic comparative studies of pasteurella infection in rabbits / Eman Mahmoud Abdelkader Mahmoud ; Supervised Nashwa Abdelsalam Ezz Eldin , Mahmoud Dardiry Elhariri , Aisha Ragab Ali

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Eman Mahmoud Abdelkader Mahmoud

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TextLanguage: English Publication details: Cairo : Eman Mahmoud Abdelkader Mahmoud , 2018Description: 103 P. : charts , facimiles ; 25cmOther title:

دراسات جينيه مقارنه على عدوى الباستيريلا فى الارانب [Added title page title]

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Dissertation note: Thesis (M.Sc) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology (Bacteriology, Immunology and Mycology) Summary: In current study, 135 samples were collected from clinically diseased rabbits of different ages from private farms of different Governorates of Egypt. The samples were collected aseptically from rabbits suffering from various signs of respiratory disorders (nasal discharges, sneezing, coughing, conjunctivitis and other respiratory disorders(. All samples were subjected to bacteriological examination, biochemical identification and molecular characterization of the isolates using Conventional PCR. 32 out of 135 samples (23.7%) were positive for P. multocida and 34 out of 135 samples (25.18%) were positive for M. haemolytica by conventional bacteriological method. PCR was confirmed that all isolates were P. multocida, and by molecular capsular typing found that all isolates were belonged to serpogroup A, the positive isolates by PCR subjected to antibiotic sensitivity testing, and the drug of choice was gentamicin. It was selected 3 strains for 16s rRNA gene sequencing and it showed genetic relation to different P . multocida subspp. with identity % = 99% . Phyloenetically, the 3 sequenced 16s rRNA gene of the Egyptian P. multocida in this study were separated into 2 different clades, the sequence with accession no. MH703779 locates in clade D that is related to the Chinese strain with accession no. EU918692. while, the 2 other sequences of the Egyptian P. multocida with accession no. MH703781 and MH703782 are related to clade C

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Holdings
Item type	Current library	Home library	Call number	Copy number	Status	Date due	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.10.10.M.Sc.2018.Em.G (Browse shelf(Opens below))		Not for loan		01010110077961000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.10.10.M.Sc.2018.Em.G (Browse shelf(Opens below))	77961.CD	Not for loan		01020110077961000

Thesis (M.Sc) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology (Bacteriology, Immunology and Mycology)

In current study, 135 samples were collected from clinically diseased rabbits of different ages from private farms of different Governorates of Egypt. The samples were collected aseptically from rabbits suffering from various signs of respiratory disorders (nasal discharges, sneezing, coughing, conjunctivitis and other respiratory disorders(. All samples were subjected to bacteriological examination, biochemical identification and molecular characterization of the isolates using Conventional PCR. 32 out of 135 samples (23.7%) were positive for P. multocida and 34 out of 135 samples (25.18%) were positive for M. haemolytica by conventional bacteriological method. PCR was confirmed that all isolates were P. multocida, and by molecular capsular typing found that all isolates were belonged to serpogroup A, the positive isolates by PCR subjected to antibiotic sensitivity testing, and the drug of choice was gentamicin. It was selected 3 strains for 16s rRNA gene sequencing and it showed genetic relation to different P . multocida subspp. with identity % = 99% . Phyloenetically, the 3 sequenced 16s rRNA gene of the Egyptian P. multocida in this study were separated into 2 different clades, the sequence with accession no. MH703779 locates in clade D that is related to the Chinese strain with accession no. EU918692. while, the 2 other sequences of the Egyptian P. multocida with accession no. MH703781 and MH703782 are related to clade C

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