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Studies on in vitro production and biological activity of bioactive substances from cleome droserifolia endemic in Egypt / Eman Omar Abdelfattah Mohammed Fathalla ; Supervised Amr saad Mohamed , Ahmad Ibrahim Amin , Maha Zaki Rizk

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Eman Omar Abdelfattah Mohammed Fathalla , 2018Description: 129 P. : charts , photoghrahs ; 25cmOther title:
  • دراسات على الانتاج المعملى والنشاط البيولوجي للمواد الفعالة فى نبات السموه المتوطن فى مصر [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry Summary: These studies were conducted at Therpautical Chemistry department , Pharmaceutical and Drug Industry Division and the department of Plant Biotechnology, Genetic Engineering and Biotechnology, National Research Center, Cairo, Egypt. The present investigation was conducted to address the following objectives: Establishment of plant tissue culture system of Cleome droserifolia and the possibility of using in vitro cell cultures in the production of bioactive substances and determine the hypoglycemic, oxidative stress markers and hepatoprotective effect of methanolic crude callus extract of Cleome droserifolia in STZ induced diabetic rats and normal rats.The most important outcomes of these studies are as follows: 1- Effect of different growth regulators (auxins and cytokinins) in production of calli cultures from different explants of cleome droserifolia The frequencies of callus formation differed significantly among different combinations of growth regulators and different explants. MS media accompanied with 0.5 mg/l NAA +1 mg/l BA (M3) showed the highest mean of callus formation, then MS media supplemented with 1 mg/l BA (M5) in both hypocotyl and root explants. The maximum of callus percentage was observed with root explants, while MS media supplemented with 1 mg/l BA (M5) showed the highest mean of embryogenic callus, hypocotyl explants produced the highest callus fresh and dry weights followed by the root
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.02.M.Sc.2018.Em.S (Browse shelf(Opens below)) Not for loan 01010110078130000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.02.M.Sc.2018.Em.S (Browse shelf(Opens below)) 78130.CD Not for loan 01020110078130000

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry

These studies were conducted at Therpautical Chemistry department , Pharmaceutical and Drug Industry Division and the department of Plant Biotechnology, Genetic Engineering and Biotechnology, National Research Center, Cairo, Egypt. The present investigation was conducted to address the following objectives: Establishment of plant tissue culture system of Cleome droserifolia and the possibility of using in vitro cell cultures in the production of bioactive substances and determine the hypoglycemic, oxidative stress markers and hepatoprotective effect of methanolic crude callus extract of Cleome droserifolia in STZ induced diabetic rats and normal rats.The most important outcomes of these studies are as follows: 1- Effect of different growth regulators (auxins and cytokinins) in production of calli cultures from different explants of cleome droserifolia The frequencies of callus formation differed significantly among different combinations of growth regulators and different explants. MS media accompanied with 0.5 mg/l NAA +1 mg/l BA (M3) showed the highest mean of callus formation, then MS media supplemented with 1 mg/l BA (M5) in both hypocotyl and root explants. The maximum of callus percentage was observed with root explants, while MS media supplemented with 1 mg/l BA (M5) showed the highest mean of embryogenic callus, hypocotyl explants produced the highest callus fresh and dry weights followed by the root

Issued also as CD

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