Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

This study was performed to investigate the microbiological quality of some hospital meals and its effective surrounding environments. Two hundred raw food items and ready to eat meals were collected from3 kitchens dish out rooms and two cafeterias of hospitals compound at Cairo. The samples were kindly withdrawn from hospitals compound at Cairo governorate, Egypt during the period of August 2019 until February 2020. Thirty-seven hand of worker swabs and fifty samples of work surfaces were collected. The samples were taken according to international standard (ISO 18593:2004). The results showed that the means log values for total viable counts were 4.62±0.28, 3.68±0.14, 2.57±0.15 and 3.25±0.17, for examined raw food samples, ready- to-eat samples, hand worker swabs and food contact surface swabs, respectively, while total coliforms count were 2.31±0.21, 1.83±0.37, 1.67±0.07 and 1.52±0.11 and total staphylococcal counts were 4.30±0.07, 2.70±0.33, 1.39 ±0.29 and 3.8±0.17, respectively. All isolated Staphylococcus aureus were positive for the virulence genes hlg and icaD genes, while E. coli isolates were positive for iss and tsh genes. The Klebsiella isolates were found to harbor fimH gene (type1) gene but only one isolate was positive for both fimH and magA gene. Pseudomonas spp. isolates were positive for both toxA and fliC genes

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