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Ex. vivo study on somatic stem cells isolated from rat liver / Marwa Mahmoud Saleh ; Supervised Motawa E. Elhouseini , Fared A. Kashawaa , Tamer A. Alshafie

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Marwa Mahmoud Saleh , 2019Description: 130 P. : charts , facsimiles ; 25cmOther title:
  • دراسه تجريبيه على الخلايا الجزعيه المعزوله من كبد الفئران [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Cancer Biology Summary: Background: Acute liver injury disorder contributes to liver fibrosis and cirrhosis which is very dangerous that may lead to hepatic cellular carcinoma (HCC) which is a common cancer worldwide. Stem cell-based therapy is considered a key area of research in end stage liver injury & diseases. Aim: This study aimed to elucidate the efficiency of different techniques to isolate non-parenchymal liver cells (NPCs) that contain stem cells from liver tissue through differential centrifugation and density gradient centrifugation, to characterize liver stem cells by flow cytometry using CD44& CD90, and, to evaluate the expression of Ý-catenin, Smad 2,3,7 genes in liver tissue using RT-PCR and to study the effect of curcumin and taurine on liver stem cells. Results: Cultured liver stem cells showed epithelial-like morphology at day 5-14. Liver stem cell markers significantly increased at day 12 and they were significantly higher in injured stem cells than non-injured-derived stem cells. Ý-catenin and Smad 2,3,7gene showed positive expression in injured liver tissues while no expression was detected in non-injured liver tissues. After curcumin and taurine treatments (5 , 400oM) for 24 h, a significant decrease in both CD90 and CD44 expression in injured-derived stem liver cells, whereas non-significant change was detected in the expression of both markers in non-injured-derived liver stem cells
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.19.02.Ph.D.2019.Ma.E (Browse shelf(Opens below)) Not for loan 01010110080532000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.19.02.Ph.D.2019.Ma.E (Browse shelf(Opens below)) 80532.CD Not for loan 01020110080532000

Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Cancer Biology

Background: Acute liver injury disorder contributes to liver fibrosis and cirrhosis which is very dangerous that may lead to hepatic cellular carcinoma (HCC) which is a common cancer worldwide. Stem cell-based therapy is considered a key area of research in end stage liver injury & diseases. Aim: This study aimed to elucidate the efficiency of different techniques to isolate non-parenchymal liver cells (NPCs) that contain stem cells from liver tissue through differential centrifugation and density gradient centrifugation, to characterize liver stem cells by flow cytometry using CD44& CD90, and, to evaluate the expression of Ý-catenin, Smad 2,3,7 genes in liver tissue using RT-PCR and to study the effect of curcumin and taurine on liver stem cells. Results: Cultured liver stem cells showed epithelial-like morphology at day 5-14. Liver stem cell markers significantly increased at day 12 and they were significantly higher in injured stem cells than non-injured-derived stem cells. Ý-catenin and Smad 2,3,7gene showed positive expression in injured liver tissues while no expression was detected in non-injured liver tissues. After curcumin and taurine treatments (5 , 400oM) for 24 h, a significant decrease in both CD90 and CD44 expression in injured-derived stem liver cells, whereas non-significant change was detected in the expression of both markers in non-injured-derived liver stem cells

Issued also as CD

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