Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Microbiology

This study included the following points: The first one concerning isolation and identification of plant growth promoting rhizobacteria (PGPR). Twelve bacterial isolates were isolated from rhizosphere of taxodium seedlings. Data revealed that six isolates were cocci, three isolates were short rods, two isolates were long rods in chains and one was long rods. All isolates were Gram positive except one isolates was Gram negative bacteria. All isolates tolerate salinity up to 12000 ppm. IAA production in there isolates was ranged from 3.85 to 7.59 mg/L. Polysaccharides ranged from 0.1609 to 12.226 mg/L. The highest two producers were characterized as: G+ cocci; 7.165 mg/L IAA; 12.226 mg/L polysaccharides; followed by G- short rods, 7.592 mg/L IAA, 8.364 mg/L polysaccharides. The two isolates were identified using Biolog as Kocuria varians and Enterobacter cloacae, respectively. The second point two PGPR isolates were used with or without mycorrhiza to alleviate salt stress on seedlings of three woody tree species (Taxodium deisticum, Khaya senegalensis and Balanites aegyptiaca). Salinity levels were 0.0, 2000, 4000 and 8000 ppm. Data revealed that all Balanites aegyptiaca seedlings exposed to 8000 ppm of saline were dead while 25% were able to stilled alive at 4000 ppm when infected with mycorrhizae

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