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Copro-molecular characterization of giardia intestinalis among symptomatic/asymptomatic Egyptians / Dina Mohamed Salah Eldin Mohamed Nasr ; Supervised Nadia Aly Essa Eldib , Hebat Allah Salah A. Yousof , Kevin Tyler

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Dina Mohamed Salah Eldin Mohamed Nasr , 2015Description: 97 Leaves : charts , facsimiles ; 25cmOther title:
  • التوصيف الجزيئي لطفيل الجيارديا المعويه لدي حالات من المصريين بأعراض و بدون أعراض [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Parasitology Summary: G. intestinalis is one of the most common human enteric protozoan worldwide that affect both humans and animals. This cross-sectional study was aimed to characterize the Giardia genotypes isolated from the stool of symptomatic and asymptomatic Egyptians and compare them with demographic and clinical data. Single stool sample was collected from 389 individuals (245 complaining of gastrointestinal symptoms and 144 apparently healthy asymptomatic individuals). Microscopically 62 positive fecal Samples for G. intestinalis trophozoites and/or cysts from both symptomatic and asymptomatic cases. Positive samples were molecularly characterized by Copro-nPCR targeting beta-giardin gene, and nPCR products were analyzed by RFLP for assemblage identification. Giardia was a frequent pathogen (62/245{25.3%}) in both symptomatic (53/245{21.6%}) and asymptomatic (9/144{6.25%}). DNA of 52 from 62 (83.9%) microscopically positive samples was amplified by nPCR. There was assemblage B predominance in 43 cases (82.7%) in both study groups 36 (83.7%) of symptomatic and 7 of (16.3%) asymptomatic persons, while 9 (17.3%) samples had assemblage A. Among the studied variables, only flatulence was significantly associated with Giardia infection and assemblage B.
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.26.M.Sc.2015.Di.C (Browse shelf(Opens below)) Not for loan 01010110068558000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.26.M.Sc.2015.Di.C (Browse shelf(Opens below)) 68558.CD Not for loan 01020110068558000

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Parasitology

G. intestinalis is one of the most common human enteric protozoan worldwide that affect both humans and animals. This cross-sectional study was aimed to characterize the Giardia genotypes isolated from the stool of symptomatic and asymptomatic Egyptians and compare them with demographic and clinical data. Single stool sample was collected from 389 individuals (245 complaining of gastrointestinal symptoms and 144 apparently healthy asymptomatic individuals). Microscopically 62 positive fecal Samples for G. intestinalis trophozoites and/or cysts from both symptomatic and asymptomatic cases. Positive samples were molecularly characterized by Copro-nPCR targeting beta-giardin gene, and nPCR products were analyzed by RFLP for assemblage identification. Giardia was a frequent pathogen (62/245{25.3%}) in both symptomatic (53/245{21.6%}) and asymptomatic (9/144{6.25%}). DNA of 52 from 62 (83.9%) microscopically positive samples was amplified by nPCR. There was assemblage B predominance in 43 cases (82.7%) in both study groups 36 (83.7%) of symptomatic and 7 of (16.3%) asymptomatic persons, while 9 (17.3%) samples had assemblage A. Among the studied variables, only flatulence was significantly associated with Giardia infection and assemblage B.

Issued also as CD

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