Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agriculture Botany

This work was carried out during two successive seasons of 2014/15 and 2015/16 at Biotechnology, Phytochemistry Department and Farm of Applied Research Center of Medicinal Plants affiliated to the National Organization for Drug Control And Research(NODCR). The present work aimed to study the effect of inoculation Apium graveolens L. seeds with biofertilizers [arbuscular mycorrhizal (AM) and/or microbein and/or plants sprayed with Thidiazuron (TDZ) fertilized with half or full dose of NPK on soil microbial activity,plant characteristiecs , chemical composition.as well as seed phytochemical analysis. The most important results could be summarized as follow: The results in both seasons showed, inoculation of celery seeds with mycorrhiza and microbein under full or half NPK fertilizers increased soil microbial activity (number of AM fungi spores and its colonization % as well as hydrogenase and nitrogenase activies). Also, plant growth characteristics, chemical constituents, photosynthtic pigments, mineral nutrients and non- structural charbohydrates) as well as number of umbel and seedyield / pant were signifcantly increased with the inoculation of celery seeds with mycorrhiza and microbien under full NPK dose. Howerer, the reduction% in seed yield/plant induced from 1/2 NPK inocoulated with mycorrhiza and microbien reached 14.3% comparing with uninocoulated full NPK dose.Morever, the reduction in volatile oil yield reached 11.5% , from SDS-PAGE protein profile, no protein banding was found between 1/2 and full NPK dose. Meanwhile, three protein bands (53, 55 and 57 KDa). were found after inoculation with mycorrhiza. whereas,Two protein bands (20 and 37KDa) were found after inculation with microbein comparing with uninoculated treatments

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