Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology

Staphylococcus aureus, a ubiquitous pathogen, possesses a remarkably rapid resistance to the majority of the developed antibiotic. S. aureus has competitive advantages allowing this pathogen to survive in many ecosystems. Hence, new approaches should be adopted to limit S. aureus infections. The use of commensal bacteria is one of the suggested solutions for combating against invasive pathogens. Staphylococcus epidermidis, major human nasal and skin bacterial aerobic resident, can provide protective signature against pathogens e.g., S. aureus. The human nasal microbiota, including diverse symbiotic microorganisms, plays an important role in protecting the human against the colonization by invasive pathogens. In brief, this work aimed to exploit bacterial-bacterial competition in order to combat multidrug-resistant S. aureus by developping a set of in vitro experiments to investigate the role of S. epidermidis in inhibiting S. aureus, and explore the composition of Egyptian nasal microbiome by collecting nasal swabs from healthy volunteers representing community members and health-care workers and defining their core microbiome. Duplicate nasal samples were collected from 17 healthy Egyptian volunteers including: ten nurses, and seven community members. One sample was cultured and the nasal isolates were identified using culture-dependent methods. DNA was extracted from the other swab and the 16S rRNA gene of the samples was sequenced

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