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Some epidemiological studies on sheep and goats brucellosis in Egypt / Hend Ibrahim Mohamed Ahmed Elsharkawy ; Supervised Samia A. Ahmed , Mohamed Abdelmonem Salem , Soliman Mohammed Soliman

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Hend Ibrahim Mohamed Ahmed Elsharkawy , 2019Description: 163 P. : charts ; 25cmOther title:
  • بعض الدراسات الوبائية عن مرض البروسيلا في الأغنام والماعز في مصر [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Internal Medicine and Infectious Disease Summary: This study was carried out for evaluation of the diagnostic performance of different serological tests; tube agglutination test ( TAT ) , buffered acidified plate antigen test (BAPAT), rose bengal plate test (RBPT), immunochromatographic assay (ICA), rivanol test (RivT), indirect ELISA (iELISA) using two types of coating antigens(smooth lipopolysaccharide; S-LPS) and ( N-lauroylsarcosine-extracted antigens; SE) and complement fixation test (CFT). Sensitivity and Specificity of various techniques were estimated. The traditional serological tests failed to distinguish the vaccinated from naturally infected animals or from animals infected with yersinia. Using iELISA with extracted antigens (SE) as a coating antigen was a more accurate test to differentiate the naturally infected animals from vaccinated animals and may differentiate between infected animals and false positive reactors. Application of restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) on sera samples from seropositive animals, Rev-1 vaccinated sheep and Brucella field strain infected sheep and goats revealed that there were samples were identified as B. melitensis biovar 3 field strain and other samples were identified as B
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.09.Ph.D.2019.He.S (Browse shelf(Opens below)) Not for loan 01010110078635000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.09.Ph.D.2019.He.S (Browse shelf(Opens below)) 78635.CD Not for loan 01020110078635000

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Internal Medicine and Infectious Disease

This study was carried out for evaluation of the diagnostic performance of different serological tests; tube agglutination test ( TAT ) , buffered acidified plate antigen test (BAPAT), rose bengal plate test (RBPT), immunochromatographic assay (ICA), rivanol test (RivT), indirect ELISA (iELISA) using two types of coating antigens(smooth lipopolysaccharide; S-LPS) and ( N-lauroylsarcosine-extracted antigens; SE) and complement fixation test (CFT). Sensitivity and Specificity of various techniques were estimated. The traditional serological tests failed to distinguish the vaccinated from naturally infected animals or from animals infected with yersinia. Using iELISA with extracted antigens (SE) as a coating antigen was a more accurate test to differentiate the naturally infected animals from vaccinated animals and may differentiate between infected animals and false positive reactors. Application of restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) on sera samples from seropositive animals, Rev-1 vaccinated sheep and Brucella field strain infected sheep and goats revealed that there were samples were identified as B. melitensis biovar 3 field strain and other samples were identified as B

Issued also as CD

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