Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Histology

Background : Myocardial infarction (MI) is one of the most important causes of cardiovascular diseases in hospitalized patients.Aim of the study: was to assess the possible effect of mangosteen and mesenchymal stem cells (MSCs) in Isoproterenol (ISO)-induced MI of adult male albino rats, monitored by histological and immunohistochemical methods. Methods: Fifty-six adult male albino rats were included in this study. They were divided into: control group (GI), group II (ISO), group III (Mangosteen+ISO), group IV (ISO+MSCs), group V (Mangosteen + Isoproterenol + MSCs). ISO was given in a dose of (250mg/kg/d) SC two times, mangosteen was given orally (18mg/200gm) and MSCs (5 x106 cells in 1mL of PBS) were injected intracardiac. Rats of groups II and III were sacrificed 2 and 6 weeks after ISO injection. Rats of group IV and Vwere sacrificed 6 days and one month after MSCs injection. Myocardial sections were stained with H&E, Masson{u2019}s trichrome and immunohistochemical stain for caspase-3 and VEGF. The mean area % of collagen fibers and immunoreactivity of caspase-3 and VEGF were measured. Homing of MSCs was detected with fluorescent microscope. Results: Myocardial sections of G II revealed discontinuity and degeneration of cardiac muscle fibers, pyknotic nuclei, inflammatory cellular infiltration, congested blood vessel, increased collagen deposition, significant increase in apoptotic marker (caspase-3 immunoreactivity)

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