Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Internal Medicine and Infectious Diseases

PPR is a highly contagious viral disease affecting mainly domesticated and wild small ruminants and considered as a major obstacle in small ruminants production. Five migratory flocks in Giza governorate (moatamadia village, zenien, saft Ellabn and kafrberak Elkhiam) and 5 sheep admitted to the clinic of faculty of veterinary medicine, Cairo university were suspected to be affected with PPR during the period from October 2014 to November 2014. A total number of 20 diseased animals (19 sheep and one goats) and 1 dead sheep belonged to the affected flocks and the five admitted cases were subjected to field diagnosis (history, general clinical examination and PM examination). A total number of 40 samples (21 buffy coats, 18 nasal swabs and one spleen tissue) were obtained from these animals for confirmation of this suspicion by virus isolation on Vero cells and RT - PCR using primers directed to the highly conserved sequence in nucleoprotein gene of PPRV. The total number of samples which gave positive results was 15 and 37 samples by VI and RT-PCR, respectively. These results confirmed the suspicion that PPRV was the causative agent. In another study a survey on PPR antibodies in sheep and goats in Giza governorate, Egypt, during the period from May 2014 to July 2015 to determine the period prevalence by SNT and c- ELISA. A total number of 316 animals (200 sheep and 116 goats) were randomly sampled for this study (one serum sample obtained from each animal). The overall seroprevalence in both animal species was determined as 67.4% (95% CI, 62.2%- 69.9%) and 65.3 % (95% CI, 62.6% - 68%) by SNT and c- ELISA, respectively. The prevalence was higher in sheep than in goats as follow: 71 % and 61 % in sheep and goats, respectively by SNT and 68% and 51.7% in sheep and goats, respectively, by c- ELISA, but this difference was statistically non-significant

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