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Construction of recombinant baculovirus expressing HA protein of Egyptian H5N1 AIV under control of whispovirus ie - 1 promoter / Mohamed Rasheed Abdelaleem Mohamed ; Supervised Hussein Aly Hussein Ahmed , Ayman Hany Mahmoud Metwally Eldeeb

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Mohamed Rasheed Abdelaleem Mohamed , 2014Description: 203 P. : facsimiles ; 25cmOther title:
  • بناء فيروس باكيلو مدمج يعبر عن بروتين الهيماجلوتينين لفيروس إنفلونزا الطيور المصرى إتش٥إن١ تحت تأثير المحفز الآنى-١ لفيروس التبقع الأبيض بالجمبرى [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology Summary: In the present study whispovirus immediate early 1 promoter (ie - 1) was used to initiate surface expression of hemagglutinin (HA) of of Egyptian H5N1 avian influenza virus (AIV). (HA) gene and whispovirus ie - 1 promoter sequence was synthesized as expression cassette (ie1 - HA) and cloned into pFastBac - 1 transfer vector of Bac - To - Bac baculovirus expression system then transformed into DH10Bac competent cells for generation of recombinant bacmid via site specific transposition, the recombinant bacmid was used for transfection of spodoptera frugiperda (Sf9) insect cells for construction of recombinant virus and expression of HA protein of H5N1 AIV. The recombinant glycoprotein expressed in Sf-9 cells showed hemadsorption activity, hemagglutinating (HA) activity was also detected in both extra - and intra - cellular recombinant HA
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.17.M.Sc.2014.Mo.C (Browse shelf(Opens below)) Not for loan 01010110064076000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.17.M.Sc.2014.Mo.C (Browse shelf(Opens below)) 64076.CD Not for loan 01020110064076000

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology

In the present study whispovirus immediate early 1 promoter (ie - 1) was used to initiate surface expression of hemagglutinin (HA) of of Egyptian H5N1 avian influenza virus (AIV). (HA) gene and whispovirus ie - 1 promoter sequence was synthesized as expression cassette (ie1 - HA) and cloned into pFastBac - 1 transfer vector of Bac - To - Bac baculovirus expression system then transformed into DH10Bac competent cells for generation of recombinant bacmid via site specific transposition, the recombinant bacmid was used for transfection of spodoptera frugiperda (Sf9) insect cells for construction of recombinant virus and expression of HA protein of H5N1 AIV. The recombinant glycoprotein expressed in Sf-9 cells showed hemadsorption activity, hemagglutinating (HA) activity was also detected in both extra - and intra - cellular recombinant HA

Issued also as CD

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