Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Andrology and S.T.Ds

The aim of the study is to compare the aseptic technology of cryoprotectant-free vitrification of human spermatozoa in large volume, to conventional freezing protocol as regards post thawing motility, vitality and sperm DNA fragmentation .Patients and methods: This study was carried out Sample of 20 male patients seeking seminal fluid analysis at the andrology laboratory of a specialized IVF center (ADAM International Hospital for Fertility and Sterility, Giza, Egypt), with the diagnoses of normozoospermia, oligozoospermia. Patients were divided into two groups Group A; samples with the diagnosis of normozoospermia, mild or moderate oligozoospermia. (Spermatozoa count more than 5x106/ml). Group B; samples with the diagnosis of severe oligozoospermia (count of or less than 5x106/ml down to at least 1 motile sperm after centrifugation).. Results: Motility of vitrified spermatozoa (in a large volume (300 æl) in the absence of permeable cryoprotectants displayed significant statistically lower levels as compared to conventional Sperm Freezing ,Vitality of vitrified spermatozoa showed significant statistically lower levels as compared to conventional Sperm Freezing, DNA fragmentation of vitrified spermatozoa showed higher levels as compared to conventional slow freezing but statistically non significant,. Conclusion: vitrification technique was quite far away from comparison with slow conventional freezing protocol, and still need further modifications

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