Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology

Background: Epidemiological studies have proved an association between drinking water sources and the incidence of H. pylori and Campylobacter spp. infection in developing countries. Aim: To investigate the role of water reservoirs as a potential source of H. pylori and Campylobacter spp. infection in Giza area, Egypt. Methods: A total of 125 water samples, from drinking water reservoirs, were collected from 13 districts of Giza governorate, Egypt. Physicochemical properties of the collected water samples including turbidity, color, odor, pH and chloride ion were determined. The filters obtained from the filtration of water samples were inoculated on a panel of different culture media including selective columbia blood agar (SCBA), modified columbia urea agar (MCUA), modified charcoal cefoperazone deoxycholate agar (mCCDA); the recovered isolated colonies were further identified by confirmatory biochemical reactions, Microscan WalkAway system or MALDI-TOF MS. Water samples were cultured in selective brain heart infusion broth (BHIB) supplemented with 20% horse serum, and selective Bolton broth; genomic DNA was extracted from the incubated cultures using phenol/chloroform/isoamyl alcohol extraction method. DNeasy® PowerWater® Kit for genomic DNA extraction from filtered water samples was used. PCR was used for the detection of H. pylori by virulence genes namely: glmM, ureA, cagA, vacA and Campylobacter spp. by cadF gene. All PCR amplified products were further sequenced

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