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Isolation and differentiation of myoblasts from human umbilical cord stem cells and muscles / Rehab Hamdy Mahmoud Mabrouk ; Supervised Mahmoud Ahmed Amer , Abeer Ahmed Bahnasy , Mervat Mohamed fouad Eldeftar

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Rehab Hamdy Mahmoud Mabrouk , 2015Description: 104 P. : facsimiles ; 25cmOther title:
  • عزل وتميز الخلايا المخلة للعضلات من الخلايا الجزعية فى الحبل السرى ومن العضلات فى الانسان [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology Summary: This study was carried out to compare myogenic differentiation of skeletal muscle explants and cord blood stem cells. I- In this study, 15 samples of umbilical cord blood were obtained, isolation of mesenchymal stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were propagated in vitro, the myogenic differentiation medium was added. The cells showed a change in their morphology myotube appeared. II- Twenty five samples of skeletal muscle (1x1 cm3) were obtained and minced using micro- explant technique .In vitro expansion was performed. Muscle precursor cells reached (75%-80%) confluence; cells were sub-cultured till myotube formation. III. RNA extraction was performed; RNA was transformed to (cDNA) and expression of Desmin and Myo D genes were assessed using RT-PCR technique.
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.M.Sc.2015.Re.I (Browse shelf(Opens below)) Not for loan 01010110068166000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.M.Sc.2015.Re.I (Browse shelf(Opens below)) 68166.CD Not for loan 01020110068166000

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology

This study was carried out to compare myogenic differentiation of skeletal muscle explants and cord blood stem cells. I- In this study, 15 samples of umbilical cord blood were obtained, isolation of mesenchymal stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were propagated in vitro, the myogenic differentiation medium was added. The cells showed a change in their morphology myotube appeared. II- Twenty five samples of skeletal muscle (1x1 cm3) were obtained and minced using micro- explant technique .In vitro expansion was performed. Muscle precursor cells reached (75%-80%) confluence; cells were sub-cultured till myotube formation. III. RNA extraction was performed; RNA was transformed to (cDNA) and expression of Desmin and Myo D genes were assessed using RT-PCR technique.

Issued also as CD

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