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Loop-mediated isothermal amplification (LAMP) assay for rapid detection of Mycoplasma pneumoniae infection in paediatric Egyptian patients / Samar Ahmed Mohamed Altohamy ; Supervised Eiman Mohammed Abdulrahman , Amal Mohammed Sayed , Reham Hamed Abdelaziz

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Samar Ahmed Mohamed Altohamy , 2018Description: 180 P. : charts , facsimiles ; 25cmOther title:
  • للكشف السريع عن عدوى المفطوره الرئوية في مرضى الأطفال المصريين المصابين بالالتهاب الرئوى LAMP فحص التضخيم متساوى الحرارة بوساطه حلقه [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology Summary: Currently, three methods are available for the routine diagnosis of infections caused by M. pneumoniae: culture, serology and nucleic acid amplification techniques. Culture is time-consuming, taking several weeks to produce results and is relatively insensitive (Loens et al., 2003b). Serological methods are insufficiently sensitive and require paired serum samples from the acute and convalescent phases of the disease, thus only allowing a retrospective diagnosis (Zhang et al., 2011). More rapid, higher sensitivity methods were therefore developed, one of them being the PCR for fragments of the P1 gene (Chaudhry et al., 2013). Previous studies described real-time PCR methods for the diagnosis of M. pneumoniae e.g. the 16S rRNA genes or the P1 adhesion gene (Hardegger et al., 2000). Also, ADP-ribosylating toxin gene encoding the CARDS (community-acquired respiratory distress syndrome) toxin for real-time PCR detection
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.Ph.D.2018.Sa.L (Browse shelf(Opens below)) Not for loan 01010110077337000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.Ph.D.2018.Sa.L (Browse shelf(Opens below)) 77337.CD Not for loan 01020110077337000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Currently, three methods are available for the routine diagnosis of infections caused by M. pneumoniae: culture, serology and nucleic acid amplification techniques. Culture is time-consuming, taking several weeks to produce results and is relatively insensitive (Loens et al., 2003b). Serological methods are insufficiently sensitive and require paired serum samples from the acute and convalescent phases of the disease, thus only allowing a retrospective diagnosis (Zhang et al., 2011). More rapid, higher sensitivity methods were therefore developed, one of them being the PCR for fragments of the P1 gene (Chaudhry et al., 2013). Previous studies described real-time PCR methods for the diagnosis of M. pneumoniae e.g. the 16S rRNA genes or the P1 adhesion gene (Hardegger et al., 2000). Also, ADP-ribosylating toxin gene encoding the CARDS (community-acquired respiratory distress syndrome) toxin for real-time PCR detection

Issued also as CD

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