Probiotics and bacteriological aspect of bovine genital tract / Seham Zaher Bottros Bebawi ; Supervised Wagih A. Gad Elsaid , Sabry D. Morgan , Mohamed M. Mohamed Elgarhy

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

The recent increase in the number and severity of cases of bovine genital disorders has prompted in the use of probiotics for the prevention and control of this disease. A systemic review was performed to assess the effectiveness of probiotic therapy. Data was extracted on the number of cases in 2 farms to receive probiotic. The study was applied on two groups, treated group (no.=103) and control group (no.=10). A total of one hundred and three cows and buffaloes with endometritis or cervicitis or vaginitis were supplied with antibiotic-free ration before the beginning of the experiment. Out of 103 examined female animals 2Cows and buffaloes3, 173 bacterial isolates were secured include (103/173) Gram-positive micro-organisms [68 isolates staphylococcus spp. and 35 isolates Streptococcus spp.] in addition to 70 isolates were Gram-negative bacilli identified as escherichia coli. Isolates were characterized and identified using the most important conventional biochemical tests as catalase test, coagulase test, nitrate reduction test, urea hydrolysis, oxidase test, gelatin liquefaction test, sugar fermentation test in addition to IMVIC test and triple sugar iron. Experimental oral administration of probiotic bacteria Lactobacillus acidophilus to the treated group were performed after the first day of genital swabbing, in a dose of 20g before meal per day per animal divided on two meals while the control group was fed their diet only. Oral administration of a probiotic preparation was shown to be effective in improving bovine endometritis; cervicitis and vaginitis in addition to enhance reproductive performance and fertility. The choice of probiotic bacteria; optimal dose and timing of administration have yet to be determined

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